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a dmdC2 mutant (SPO3804::Tn5) can not grow on 3-(methylthio)propanoate as the sole source of carbon, indicating that this pathway is essential for growth on 3-(methylthio)propanoate. In contrast, the mutant grows similarly to wild-type with 3-(dimethylsulphonio)propanoate, indicating that the cleavage pathway, initiated by DddQ or DddP13, remains capable of supporting growth
in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min*mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increase during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism
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