EC Number   |
General Information  |
Reference |
|---|
    1.3.1.33 | evolution |
all modern sequences of light-dependent protochlorophyllide oxidoreductase POR diverged from a single sequence about 1.36 billlion years ago. The LPOR gene was then duplicated at least 10 times in angiosperms, leading to the formation of two or even more LPOR isoforms in multiple species. In the case of Arabidopsis thaliana, isoforms PORA and PORB originated in one duplication event, in contrary to the isoform PORC, which diverged first |
762710 |
| 1.3.1.33 | evolution |
DPOR (EC 1.3.7.7) and LPOR (EC 1.3.1.33) initially evolved in the ancestral prokaryotic genome perhaps at different times. DPOR originated in the anoxygenic environment of the Earth from nitrogenase-like enzyme of methanogenic archaea. Due to the transition from anoxygenic to oxygenic photosynthesis in the prokaryote, the DPOR was mostly inactivated in the daytime by photosynthetic O2 leading to the evolution of oxygen-insensitive LPOR that could function in the light. The primary endosymbiotic event transferred the DPOR and LPOR genes to the eukaryotic phototroph, the DPOR remained in the genome of the ancestor that turned into the plastid, whereas LPOR was transferred to the host nuclear genome. Despite the evolution of its nonhomologous isofunctional counterpart LPOR, the DPOR continues to be functional in both oxygenic and anoxygenic photosynthetic organisms. Thus, DPOR was not exactly replaced but supplemented with the LPOR. Limnohabitans sp. strain 15K has acquired LPOR through horizontal gene transfer |
763678 |
| 1.3.1.33 | evolution |
DPOR (EC 1.3.7.7) and LPOR (EC 1.3.1.33) initially evolved in the ancestral prokaryotic genome perhaps at different times. DPOR originated in the anoxygenic environment of the Earth from nitrogenase-like enzyme of methanogenic archaea. Due to the transition from anoxygenic to oxygenic photosynthesis in the prokaryote, the DPOR was mostly inactivated in the daytime by photosynthetic O2 leading to the evolution of oxygen-insensitive LPOR that could function in the light. The primary endosymbiotic event transferred the DPOR and LPOR genes to the eukaryotic phototroph, the DPOR remained in the genome of the ancestor that turned into the plastid, whereas LPOR was transferred to the host nuclear genome. Despite the evolution of its nonhomologous isofunctional counterpart LPOR, the DPOR continues to be functional in both oxygenic and anoxygenic photosynthetic organisms. Thus, DPOR was not exactly replaced but supplemented with the LPOR. LPOR protein phylogeny further corroborates the horizontal gene transfer from cyanobacteria |
-, 763678 |
| 1.3.1.33 | evolution |
oxygen-sensitive dark-operative NADPH:Pchlide oxidoreductase enzyme (DPOR) and light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) show very low sequence homology. In most organisms they occur simultaneously. However, angiosperms lack LPOR and became unable to synthesize chlorophyllides and chlorophylls (Chls) in the absence of light |
763100 |
| 1.3.1.33 | malfunction |
a PORA null mutant (porA-1) and PORA RNAi lines display severe photoautotrophic growth defects, which can be partially rescued on sucrose-supplemented growth media. Elimination of PORA during skotomorphogenesis results in reductions in the volume and frequency of prolamellar bodies, and in photoactive Pchlide conversion |
726185 |
| 1.3.1.33 | malfunction |
an enzyme-less mutant grows photoautotrophically in moderate light and contains a maximum of 20% of the wild type chlorophyll level |
726255 |
| 1.3.1.33 | malfunction |
it is shown that a porA-1 null mutant (porA mutant) carries a second dissociation insertion in another gene closely linked to the PORA gene that is expected to affect the phenotype of the porA mutant |
726188 |
| 1.3.1.33 | malfunction |
it is shown that an Arabidopsis thaliana porB-1 porC-1 double mutant can be functionally rescued by the addition of ectopically expressed PORA, which suffices in the absence of either PORB or PORC to direct bulk chlorophyll synthesis and normal plant development |
726185 |
| 1.3.1.33 | malfunction |
map-based cloning of the faded green leaf (fgl) locus in Oryza sativa is performed, and reveals that fgl harbors a 1-bp deletion in the coding region of OsPORB, resulting in a frameshift mutation and premature translational termination. Mutant is complemented by OsPORB |
726175 |
| 1.3.1.33 | malfunction |
overexpression of PORC in Arabidopsis thaliana reduces the accumulation of protochlorophyllide in high light-grown plants that results in minimal generation of 1O2 and plants are protected from 1O2-mediated oxidative damage caused by high light. PORC overexpression protects the plants from oxidative herbicidal action of 5-aminolevulinicacid. Overexpression of PORC results in coordinated upregulation of gene/protein expression of several Chl biosynthetic pathway enzymes resulting in enhanced Chl synthesis in light-grown plants |
726283 |
