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EC Number
General Information
Commentary
Reference
metabolism
according to a model for the maturation of PceA, the pce genes are transcribed in tetrachloroethene-grown cells and the PceA cofactor-free precursor is formed and binds to the PceT chaperone. When corrinoid cofactor is provided by de novo biosynthesis, it is incorporated into prePceA. After incorporation of this cofactor and assembly and incorporation of the iron-sulfur clusters, the precursor protein is correctly folded and exported to the exoplasm by the Tat machinery. After cleavage of the signal peptide, the protein is bound to PceB, which may serve as a membrane anchor for PceA. In cells subcultivated for a few steps with fumarate instead of tetrachloroethene, corrinoid biosynthesis is impeded. This causes aggregation of the prePceA together with PceT and other proteins inside the cells. Excision of the pce gene cluster occurs upon longterm cultivation with fumarate. The loss of the gene cluster is delayed in the presence of exogenous vitamin B12
physiological function
the strain shows a close coupling of cell growth and chlorine removal during tetrachloroethene dechlorination
physiological function
overview on dehalorespiration with H2 as electron donor and tetrachloroethene as electron acceptor. Tetrachloroethene reducing organisms include Desulfitobacterium sp., Dehalobacter restrictus as well as Dehalospirillum multivorans, Desulfuromonas chloroethenica, Dehalococcoides ethenogenes, and the facultative anaerobes Enterobacter strain MS-1 and Enterobacter agglomerans
Results 1 - 3 of 3