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Results 1 - 6 of 6
EC Number
General Information
Commentary
Reference
physiological function
bacterial luciferase requires an exogenous source of reduced flavin mononucleotide for bioluminescence activity. There is no stable complex formation between luciferase and oxidoreductases Fre/NfsA from Escherichia coli or Frp from Vibrio harveyi, which are believed to provide reduced flavin for luciferase activity. No difference in the levels of luciferase expression in either the DELTAfre or DELTANsfA strains
physiological function
expression in Staphylococcus aureus Xen29. In the absence of antibiotics, staphylococcal bioluminescence increases over time until a maximum after ca. 6 h of growth, and subsequently decreases to the detection threshold after 24 h of growth. Up to minimal inhibitory concentrations of the antibiotics vancomycin, ciprofloxacin, erythromycin or chloramphenicol, bioluminescence increases according to a similar pattern up to 6 h of growth, but after 24 h, bioluminescence is higher than in the absence of antibiotics. Antibiotic pressure impacts the relation between bioluminescence per organism and bioluminescence. Under antibiotic pressure, bioluminescence is not controlled by luxA expression but by cofactors impacting the bacterial metabolic activity
physiological function
isolated dimeric LuxF binds four molecules of the derivatized flavin, 6-(3'-(R)-myristyl)-FMN. LuxF binds myrFMN tightly with a dissociation constant of 80 nM
physiological function
luciferase is the enzyme responsible for light emission in bioluminescent bacteria, it catalyzes the reaction of reduced flavin mononucleotide FMNH2, O2, and an aliphatic aldehyde to yield FMN, the corresponding carboxylic acid, and blue-green light
physiological function
the luxCDABE encoded protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations
physiological function
transient and stable transfection of human kidney, breast cancer, and colorectal cancer cell lines by a codon optimized lux expression cassette using viral 2A elements as linker regions. The expression product produces autobioluminescent phenotypes in all cell lines tested without the induction of cytotoxicity and allows for repeated interrogation of populations and self-directed control of bioluminescent activation with detection limits and EC50 values similar to traditional reporter systems
Results 1 - 6 of 6