EC Number   |
General Information |
Reference |
|---|
   1.14.11.26 | more |
additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview |
-, 725639 |
| 1.14.11.26 | metabolism |
deacetylcephalosporin C synthase (DACS) transforms an inert methyl group of deacetoxycephalosporin C (DAOC) into an active hydroxyl group of deacetylcephalosporin C (DAC) during the biosynthesis of cephalosporins |
-, 744105 |
| 1.14.11.26 | more |
homology modeling of DACS structure |
-, 744105 |
| 1.14.11.26 | evolution |
the enzyme belongs to the family of 2-oxoglutarate-dependent oxygenases |
-, 744105 |
| 1.14.11.26 | metabolism |
the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains |
-, 725639 |
| 1.14.11.26 | physiological function |
the enzyme is involved in the biosynthesis of cephalosporins, it carries out a critical transformation of an inert cephem methyl group of deacetoxycephalosporin C (DAOC) into an active hydroxyl group of deacetylcephalosporin C (DAC) in the presence of iron, oxygen, ascorbic acid, and 2-oxoglutarate. It is a step which is chemically difficult to accomplish and which offers a second substitution site for derivatization in cephalosporins |
-, 744105 |
| 1.14.11.26 | metabolism |
two enzymes, deacetoxycephalosporin C synthase and deacetoxycephalosporin C hydroxylase, are involved in the conversion of penicillin N into deacetylcephalosporin C in Streptomyces clavuligerus |
-, 725639 |
