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EC Number Expression Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22down Hfq represses IS10/Tn10 transposase expression through both antisense RNA-dependent and independent mechanisms. Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation, Hfq binding to site 1 is critical for regulation of transposase expression in vivo. An interaction between the distal surface of Hfq and the ribosome-binding site of transposase mRNA (RNA-IN) is required for repressing translation initiation. Hfq represses transposase translation by facilitating antisense pairing as well as through an antisense-independent mechanism, overview. 3.2Fold decrease in transposase expression in the presence versus the absence of Hfq 739105
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more impact of mutant forms of Hfq on IS10 transposase expression and transposition, Hfq binding of native RNA-IN in vivo is inhibited by site 1 mutations, overview 739105
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22up the small RNA ChiX activates transposase expression by titrating Hfq away from transposase mRNA, RNA-IN. Overexpression of ChiX results in a 4.5fold reduction in the amount of RNA-IN associated with Hfq. ChiX positively regulates IS10 transposase translation. ChiX competes with RNA-IN for Hfq-binding in vivo 739105
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