EC Number   |
Reaction |
Reference |
|---|
    5.4.4.2 | chorismate = isochorismate |
- |
- |
| 5.4.4.2 | chorismate = isochorismate |
general acid-general base catalysis with a lysine as the base and a glutamic acid as the acid, in reverse protonation states and with a conformational or binding step as the slow step |
726817 |
| 5.4.4.2 | chorismate = isochorismate |
isochorismate synthase (IS) performs a 1,5-displacement mechanism during the chorismate conversion to intermediates through the use of a nucleophile, which is water in the case of IS, but might also be possible with ammonia. The hydroxyl group in the isochorismate is generated from water and not through an intramolecular rearrangement |
747617 |
| 5.4.4.2 | chorismate = isochorismate |
isochorismate synthase performs a 1,5-displacement mechanism during the chorismate conversion to intermediates through the use of a nucleophile, which is water in the case of isochorismate synthase, but might also be possible with ammonia. The hydroxyl group in the isochorismate is generated from water and not through an intramolecular rearrangement |
747617 |
| 5.4.4.2 | chorismate = isochorismate |
K90 is the base that activates water for nucleophilic attack at the chorismate C2 carbon |
678320 |
| 5.4.4.2 | chorismate = isochorismate |
overall reaction, salicylate sythase converts chorismate into salicylate via an isochorismate intermediate. The isochorismatase activity of bifunctional enzyme MbtI requires Lys205, which nucleophilically activates a water molecule for attack on chorismate at C-6 and Glu252 that polarizes the C-4 hydroxyl-leaving group |
748448 |
