EC Number   |
Reaction |
Reference |
|---|
    4.3.1.19 | 2-aminobut-2-enoate = 2-iminobutanoate |
(1b), spontaneous |
- |
| 4.3.1.19 | 2-iminobutanoate + H2O = 2-oxobutanoate + NH3 |
(1c), spontaneous |
- |
| 4.3.1.19 | L-threonine = 2-aminobut-2-enoate + H2O |
(1a) |
- |
| 4.3.1.19 | L-threonine = 2-oxobutanoate + NH3 |
(overall reaction) |
- |
| 4.3.1.19 | L-threonine = 2-oxobutanoate + NH3 |
that from P. putida is not. The enzyme from a number of sources also acts on L-serine, cf. EC 4.3.1.17, L-serine ammonia-lyase. The reaction catalysed probably involves initial elimination of water, hence the enzyme's original classification as EC 4.2.1.16, threonine dehydratase, followed by isomerization and hydrolysis of the product with C-N bond breakage |
- |
| 4.3.1.19 | L-threonine = 2-oxobutanoate + NH3 |
The enzyme from many sources is a pyridoxal-phosphate protein |
- |
