EC Number |
Reaction |
Reference |
---|
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
- |
- |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
active site and reaction mechanism, structure-function relationship, structural coordination spheres are important, residues of the first coordination sphere involved in metal binding are indispensable for catalytic activity, Glu185 is essential for catalytic activity |
674408 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
active site architecture |
671038 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
active site structure and catalytic mechanism, modeling of the substrate ribulose 5-phosphate bound in the active site with the phosphate group anchored at the sulfate site and the placement of the ribulose group guided by the glycerol site, the catalytic reaction involves residues Asp41, Cys66, and Glu174, and the Asp99-His136 dyad |
671958 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
catalytic mechanism |
673430, 674403, 675800 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
catalytic reaction mechanism, substrate recognition and active site structure, active site consists of three glutamates, two aspartates, two histidines, and a cysteine which may provide the means for general acid and base catalysis and for coordinating the Mg2+ cofactor within the active site |
677168 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
reaction mechanism |
675576 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
reaction mechanism involving intramolecular skeletal rearrangement, a cluster of charged amino acid residues comprising Arg25, Glu26 and Glu28, Asp21 and Asp30 is essential for catalytic activity, as well as His164 and Glu185 |
674406 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
reaction mechanism, detailed overview |
-, 748186 |
4.1.99.12 | D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
reaction mechanism, the enzyme possesses an essential acidic active-site loop |
675342 |