EC Number |
Reaction |
Reference |
---|
3.7.1.3 | L-kynurenine + H2O = anthranilate + L-alanine |
A pyridoxal-phosphate protein. |
- |
3.7.1.3 | L-kynurenine + H2O = anthranilate + L-alanine |
a pyridoxal-phosphate protein. Also acts on 3'-hydroxykynurenine and some other (3-acrylcarbonyl)-alanines, hydrolytic beta,gamma-cleavage of aryl-substituted gamma-keto-alpha-amino acids |
- |
3.7.1.3 | L-kynurenine + H2O = anthranilate + L-alanine |
aldol and retro-aldol reactions |
- |
3.7.1.3 | L-kynurenine + H2O = anthranilate + L-alanine |
catalytic reaction mechanism via several intermediates, e.g. external aldimine, quinonoid, ketimine, Gem-diolate, and enamine |
733495 |
3.7.1.3 | L-kynurenine + H2O = anthranilate + L-alanine |
the proposed mechanism of the retro-Claisen reaction requires extensive acid/base catalysis, the phosphate of pyridoxal 5'-phosphate is an acid/base catalyst in the mechanism of Pseudomonas fluorescens kynureninase, Tyr226 hydrogen bonds to the phosphate of the pyridoxal 5'-phosphate cofactor. Tyr226 mediates proton transfer between the substrate and the phosphate, which accelerates formation of external aldimine and gem-diol intermediates, catalytic reaction mechanism via several intermediates, e.g. external aldimine, quinonoid, ketimine, Gem-diolate, and enamine, overview |
733832 |