3.6.1.63 | alpha-D-ribose 1-methylphosphonate 5-triphosphate + H2O = alpha-D-ribose 1-methylphosphonate 5-phosphate + diphosphate |
when the hydrolysis reaction of D-ribose-5-triphosphate catalyzed by PhnM is conducted in oxygen-18 labeled water, the oxygen-18 is found exclusively in D-ribose-5-phosphate and not in diphosphate. Therefore, water attacks the alpha-phosphoryl group of D-ribose-5-triphosphate rather than the beta-phosphoryl group |
720545 |