3.4.22.2 | Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1' |
the His-Cys catalytic diad in free papain is fully protonated, -NH(+)/-SH. The experimental pKa of 8.62 for His159 imidazole in free papain is assigned to the -NH(+)/-SH and -N/-SH equilibrium |
710502 |