EC Number   |
Reaction |
Reference |
|---|
    3.2.2.1 | a purine nucleoside + H2O = D-ribose + a purine base |
- |
- |
| 3.2.2.1 | a purine nucleoside + H2O = D-ribose + a purine base |
main features of transition state are activation of the heterocyclic base by protonation of/or hydrogen bonding to O2, an extensively broken C-N-glycosysic bond, formation of an oxocarbenium ion in the ribose ring, C3-exo ribose ring conformation, and almost no bond formation to the attacking nucleophile |
667809 |
| 3.2.2.1 | a purine nucleoside + H2O = D-ribose + a purine base |
mechanism couples loop closure to the positioning of active site residues. Relationship between the dimeric form of the enzyme and its catalytic activity |
669883 |
| 3.2.2.1 | a purine nucleoside + H2O = D-ribose + a purine base |
ordered bi-bi kinetic mechanism and high forward commitment with inosine as substrate. The dominant energetic contribution towards catalysis comes from ribosyl and water activation, leaving group activation also makes a considerable contribution. The leaving group is unlikely to be protonated prior to N-glycosidic bond cleavage and active site binding interactions to the purine leaving group are required for efficient ribosyl and/or water activation |
683183 |
