EC Number   |
Reaction |
Reference |
|---|
   3.1.30.2 | endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products |
catalytic mechanism, enzyme monomer-DNA binding complex, hydration sites and influence of solvent on structure and reaction, active site structure |
666881 |
| 3.1.30.2 | endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products |
DNA cleavage stereochemistry and mechanism involving interaction of DNA with three zinc ions, the enzyme contains a cocatalytic zinc site |
665188 |
| 3.1.30.2 | endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products |
general acid-base catalysis. The enzyme cleaves 3'- and 5'-phosphodiester bonds to form 5'-phosphorylated oligonucleotides. The hydrolysis of the phosphodiester bonds proceeds as a result of the nucleophilic displacement at the phosphorus atom. The enzyme active site is formed at least by 8 amino acid residues, Arg57, Asp86, His89, Gln114, Asn119 and Glu127. Arg57, Asp86, His89 and Glu127 are important for the catalytic activity of the enzyme. His89 is a potential candidate for the function of a general base catalyst that accepts a proton from a solvent H2O molecule, which is the attacking nucleophile. The reaction is accompanied by the inversion of configuration at the phosphorus atom and proceeds in accordance with an in-line mechanism |
657343 |
| 3.1.30.2 | endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products |
mechanism |
135016 |
| 3.1.30.2 | endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products |
one-step in line mechanism of enzymatic hydrolysis. The enzyme hydrolyzes internucleotide phosphothioate linkages of Rp configuration with inversion of configuration at the P-atom. The enzyme is stereoselective towards internucleotide phosphothioate linkages of Rp configuration. The involvement of a non-bridging oxygen in contact with a magnesium ion is a critical discriminatory factor for selectivity towards phosphothioate analogues |
654960 |
