EC Number   |
Reaction |
Reference |
|---|
   3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
mechanism, The enzyme is an ATP-dependent DNA exonuclease and a helicase, its exonuclease activity is subject to regulation by an octameric nucleotide sequence called chi. |
652076 |
| 3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
preference for double-stranded DNA, possesses DNA-dependent ATPase activity, acts endonucleolytically on single-stranded circular DNA |
133995 |
| 3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
the enzyme complex is activated once unwinding progresses. Extension of the 5'-tail of the unwound duplex induces a large conformational change in the RecD subunit, that is transferred through the RecC subunit to activate the nuclease domain of the RecB subunit. The process involves a SH3 domain that binds to a region of the RecB subunit in a binding mode that is distinct from others observed previously in SH3 domains |
750403 |
| 3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
The enzyme generates 3 single-stranded DNA ends used by RecA for homologous recombination. The exonuclease activity is altered when the enzyme encounters a Chi sequence, 5-GCTGGTGG-3, in double-stranded DNA, an event critical to generation of 3 single-stranded DNA. Chi recognition requires that Chi be flanked by DNA at either end. A specific site for Chi recognition exists on enzyme, which binds Chi with greater affinity than a non-Chi sequence and is probably adjacent to non-specific DNA binding sites. |
653394 |
| 3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
The enzyme is involved initiating DNA recombination. The nuclease properties of the enzyme are regulated upon recognition of a specific DNA sequence by the translocating enzyme. This sequence, called chi, corresponds to the octamer 5-GCTGGTGG-3. |
653270 |
| 3.1.11.5 | Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides |
The enzyme is required for homologous recombination and DNA repair, the degradative and recombinational activities of enzyme, as well as its structure, are regulated by a specific DNA sequence called Chi. The recombination requires loading of RecA by RecBCD enzyme and that the RecD subunit inhibits this reaction. |
653653 |
