EC Number   |
Reaction |
Reference |
|---|
   2.8.2.4 | 3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate |
- |
- |
| 2.8.2.4 | 3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate |
a reaction transition state with substantial S-O bond fission and partial charge neutralization of the leaving group |
674662 |
| 2.8.2.4 | 3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate |
random bi-bi mechanism catalytic mechanism of SULTs, many family members have distinct, but overlapping substrate specificities, the enzymes have a sequential catalytic mechanism that is susceptible to substrate inhibition |
676745 |
| 2.8.2.4 | 3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate |
the proposed reaction mechanism: upon binding of PAPS, Ser137 forms an H-bond with Lys47, thus prevents its interaction with the bridging oxygenin PAPS and its further hydrolysis; His107 attracts a proton from the substrate hydroxyl (i.e. of E2 or DHEA), and enables nucleophilic attack at the sulfur atom in PAPS. In the next step, Lys47 interacts with the 5' phosphate of PAPS, which helps in dissociation of the sulfuryl group and in its transfer to the substrate. Finally, the reaction products, PAP and E1-S, are released, which completes the catalytic cycle |
761169 |
