2.7.8.13 | UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol |
a one-step, single displacement mechanism. The oxyanion of the polyprenyl-phosphate attacks the b-phosphate of the nucleotide substrate, leading to the formation of product lipid I and liberation of UMP. The involvement of an invariant aspartyl residue in the deprotonation of the lipid substrate is possible. Formation of a covalent reaction intermediate |
-, 737788 |
2.7.8.13 | UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol |
in gram-negative and some gram-positive organisms the L-lysine is replaced by meso-2,6-diaminoheptanedioate (meso-2,6-diaminopimelate, A2pm), which is combined with adjacent residues through its L-centre. The undecaprenol involved is ditrans, octacis-undecaprenol |
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