EC Number   |
Reaction |
Reference |
|---|
   2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
functional role of Trp310 residue located in the small loop near the active site of the enzyme |
675284 |
| 2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
mechanism |
489523 |
| 2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
not distinguishable from EC 2.4.1.90 which has identical substrate specificities. EC 2.4.1.38/90 is identical with the A protein of EC 2.4.1.22. Terminal N-acetyl-beta-D-glucosaminyl residues in polysaccharides, glycoproteins and glycopeptides can act as acceptor. High activity is shown towards such residues in branched-chain polysaccharides when these are linked by beta-1,6-links to galactose residues, lower activity towards residues linked to galactose by beta-1,3-links. A component of EC 2.4.1.22 (lactose synthase) |
- |
| 2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
reaction mechanism and structural changes |
675370 |
| 2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
reaction mechanism, structure-function relationship, upon binding of metal ion and sugar-nucleotide, the flexible loops undergo a marked conformational change, from an open to a closed conformation. Conformational change simultaneously creates at the C-terminal region of the flexible loop an oligosaccharide acceptor binding site that did not exist before. The loop acts as a lid covering the bound donor substrate. After completion of the transfer of the glycosyl unit to the acceptor, the saccharide product is ejected and the loop reverts to its native conformation to release the remaining nucleotide moiety |
686257 |
| 2.4.1.38 | UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide |
the sequences of cDNA isolated from mammary and F9 cell lines are identical, thus indicating that EC 2.4.1.38 and EC 2.4.1.90 are non-distinguishable |
489496 |
