EC Number |
Reaction |
Reference |
---|
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
active site |
487971 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
active site on small subunit |
-, 487916, 487918, 487932 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
almost the entire glutamyl moiety is necessary for recognition in the binding site of the donor substrate |
487971 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
in the human enzyme, a specific residue in the Cys-Gly binding site plays a critical role in recognizing the Cys-Gly moiety or the acceptor molecules by interacting with the C-terminal carboxy group, whereas the Cys side chain and the Cys-Gly amide bond are not recognized significantly |
685119 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
kinetic mechanism, pH-dependence |
-, 487921 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
kinetic study |
-, 487921, 487925, 487928, 487929, 487930, 487952, 487962, 487963, 487964, 487966, 487969 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
mechanism |
-, 487921, 487923, 487925, 487929, 487930, 487931, 487954 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
ping-pong bi bi mechanism |
487907 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
structure |
-, 487916, 487931, 487939 |
2.3.2.2 | a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid |
the enzyme binds glutathione as donor substrate and initially forms a gamma-glutamyl enzyme that can then react with a water molecule or an acceptor substrate (usually an amino acid or a dipeptide) to form glutamate or a product containing a new gamma-glutamyl isopeptide bond, respectively, thus regenerating the free enzyme |
675806 |