EC Number |
Reaction |
Reference |
---|
1.5.1.33 | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ |
- |
- |
1.5.1.33 | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ |
conserved residue Tyr194 forms hydrogen bonds with the nucleophilic center of the substrate and stabilizes the transition state, conserved residue Lys198 lowers the pKa of the tyrosine, helps to stabilize the transition state, and may function as proton source for the tyrosine |
657281 |
1.5.1.33 | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ |
ordered sequential reaction mechanism |
484952 |
1.5.1.33 | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ |
ordered ternary complex mechanism with NADPH binding first and NADP+ dissociating after the reduced pteridine. The enzyme transfers the pro-S hydride of NADPH to carbon 6 on the si face of dihydrofolate |
484957 |
1.5.1.33 | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ |
the enzyme exhhibits a ping-pong mechanism |
742445 |