EC Number   |
Reaction |
Reference |
|---|
    1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
allosteric regulation of the catalytic activity of subunit R1 |
437987 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
binding of specificity effector rearranges loop 2 and moves residue P294 out of the catalytic site, accomodating substrate binding. substrate binding further rearranges loop2. Cross-talk occurs largely through R293 and Q288 of loop 2. Substrate ribose binds with its 3 hydroxyl closer than its 2 hydroxyl to residue C218 of the catalytic redox pair |
676853 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
catalytic mechanism |
686735 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
kinetics and mechanism of formation of the tyrosyl radical and micro-oxo-diiron cluster in the R2 subunit |
437989 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
NDP reduction requires cleavage of the 3'-C-H bond of the substrate, hypothesis of enzyme mechanism |
437955 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
postulated mechanism, radical cation intermediates |
437936 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
presence of divalent cations mediates a rapid radical-transfer equilibrium between W48 and Y356. Cation-mediated propagation of the radical from W48 to Y356 gives rise to a fast phase of Y radical production that is essentially coincident with W48 cation radical formation and creates an efficient pathway for decay of W48 cation radical |
672201 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
proposed mechanism |
437918, 437936, 437938, 437953 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
reaction involves formation of a keto-deoxyribonucleotide intermediate. In case of furanone inhibitors, the intermediate dissociates from the active site, depending on the solvation free energy of the 2-substituents, its influence inside the active site, and the charge transfer mechanism from a protein side chain to solution as thermodynamic driving forces. Substrates do not dissociate from the active site but complete the catalytic cycle |
677078 |
| 1.17.4.1 | 2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin |
reaction mechanism via formation of a tyrosyl radical at Tyr356 od subunit R1, model for radical transport in which there is a unidirectional transport of the electron and proton transport among residues of R1 |
687294 |
