EC Number   |
|---|
   7.1.1.10 | high grade purification of native NDH-1L complex from cells of Thermosynechococcus elongatus by nickel affinity chromatography and gel filtration |
| 7.1.1.10 | in Synechocystis sp. 6803, some hydrophilic subunits of wild-type NDH-1 complex, including NdhH-NdhK, NdhN, and NdhO, are copurified with photosystem I (PSI), and several PSI subunits, PsaB-PsaD and PsaF, are copurified with NDH-1 by Ni2+ affinity chromatography |
| 7.1.1.10 | in the NDH-1 complex, the interactions between the connecting and membrane core domains seem to be strong enough to keep them together during solubilization |
| 7.1.1.10 | native enzyme complex from membrane fraction of Synechocystis sp. PCC 6803 cells by ultracentrifugation at 150000 x g, isolation of thylakoid membranes. Recombinant His6-tagged YFP-tagged wild-type and mutant subunits NdhS from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography |
| 7.1.1.10 | native NDH-1L complex from strain BP-1 by membrane preparation through ultracentrifugation at 150000 x g, nickel affinity chromatography (owing to the native histidine-rich area within the hydrophobic subunit NdhF1), and solubilization with 1% w/v n-dodecyl-beta-D-maltoside, followed by again nickel affinity chromatography, ultrafiltration, and gel filtration |
| 7.1.1.10 | native subunits from membranes by affinity chromatography |
| 7.1.1.10 | recombinant wild-type and mutant CpcG2-YFP-His6 supercomplex from Synechocystis sp. 6803 thylakoid membranes by Ni2+ affinity chromatography |
| 7.1.1.10 | wild-type NDH-1L complex is recovered from wild-type cells of Thermosynechococcus elongatus by Ni2+ column chromatography, NDH-1S or NDH-1M is not recovered from wild-type cells by chromatography of this kind, recombinant His-tagged enzyme complex NDH-1L from Thermosynechococcus elongatus solubilized thylakoid membranes by nickel affinity chromatography |
