EC Number   |
|---|
    6.3.4.5 | - |
| 6.3.4.5 | 3 enzyme forms ASSI, ASSII, and ASSIII |
| 6.3.4.5 | affinity chromatography |
| 6.3.4.5 | analysis by native gel electrophoresis shows that argininosuccinate synthetase can bind bacterial lipopolysaccharide and lipid A |
| 6.3.4.5 | argininosuccinate synthetase is purified from mouse liver. Livers are excised from 20 male mice (C3H/He strain, age 7-8 weeks) and homogenized in 100 mM Tris-HCl (pH 7.5) containing 1 mM citrulline and 1 mM aspartic acid. Homogenate is centrifuged. After salting-out with (NH4)2SO4. The sample is subjected to anion-exchange chromatography on DE-52. Concentrated samples are loaded on a column of Sephacryl S-300 HR. The collected first peak fractions are dialyzed and subjected to anion-exchange chromatography on UNO S-1 |
| 6.3.4.5 | Ni-NTA agarose column chromatography |
| 6.3.4.5 | recombinant |
| 6.3.4.5 | recombinant ASS1 by His affinity chromatography and gel filtration |
| 6.3.4.5 | recombinant EAS |
| 6.3.4.5 | recombinant His-tagged enzyme from Escherichia coli by nickel affinty chromatography |
