EC Number |
---|
6.3.4.3 | - |
6.3.4.3 | ammonium sulfate, Phenyl Sepharose, Source15Q, hydroxyapatite, Resource Q |
6.3.4.3 | by anion-exchange chromatography |
6.3.4.3 | heparin agarose column chromatography and phenyl Sepharose column chromatography |
6.3.4.3 | large-scale purification |
6.3.4.3 | recombinant enzyme is partially purified from HEK-293 cells by subcellular fractionation |
6.3.4.3 | recombinant FTHFS |
6.3.4.3 | recombinant FTHFS, heparin Agarose, Phenyl Sepharose |
6.3.4.3 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS Rosetta by nickel affinity chromatography |
6.3.4.3 | recombinant maltose-binding protein fusion mitochondrial isozyme from Escherichia coli by metal affinity chromatography and gel filtration, the MBP-tag is cleaved off by TEV protease |