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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2-
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2by using talon metal affinity resin, a glass Econo-Column and a Sephadex G-50 column. Proteolysis is performed at 23°C for 40 min. The protein solution is then loaded again on a Talon resin column but this time the flow-through, containing CTPS without His tag, is collected. Finally the protein is loaded onto a 0.2-ml SourceQ15 medium column.
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2enzyme is purified by gel filtration on a HiLoad 26/60 Superdex 200 column. Active fractions are pooled and applied to a Mono Q HR 5/5 column. Final purifications step is performed on a HiLoad 26/60 Superdex 75 column. The isolated EcCTPS is found >90% pure as judged by SDS-PAGE and ESI-MS.
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2His6-tagged human CTP synthetase 1 is purified by loading onto a Ni2+-NTA column
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2His6-tagged wild type and mutant human CTP synthetase 1 enzymes are expressed and purified from yeast cell extracts with Ni2+-NTA resin
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2His6-tagged wild type human CTP synthetase 1 is expressed and purified from Escherichia coli by Ni2+-NTA chromatography and with PorosHQ-ion-exchange chromatography
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2Ni2+-IDA resin column chromatography
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2partial
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2protein is purified by using HisTrap HP and Superdex 75 columns
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2recombinant wild-type and mutant enzyme L109A
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