EC Number |
---|
6.2.1.14 | - |
6.2.1.14 | anion exchange, gel filtration, chromatofocusing |
6.2.1.14 | partial |
6.2.1.14 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography, tag cleavage through thrombin, and gel filtration |
6.2.1.14 | recombinant His6-tagged enzyme by nickel affinity chromatography, removal of the tag by TEV protease, another step of nickel affinity chromatography, and gel filtration, to homogeneity |