EC Number |
---|
5.6.1.1 | - |
5.6.1.1 | 90% pure after Ni2+-NTA agarose, analyzed by SDS-PAGE |
5.6.1.1 | baculovirus-expressed, recombinant DM-Kat60 |
5.6.1.1 | copurification of p81 and p60 |
5.6.1.1 | GSH-Sepharose affinity chromatography, ion exchange chromatography, cleavage with precission protease, GSH-Sepharose affinity chromatography and gel filtration |
5.6.1.1 | GST tagged p60-katanin purified using glutathione-Sepharose |
5.6.1.1 | GSTrap column chromatography, GST tag cleaved with PreScission Protease, GST-Sepharose chromatography, gel filtration |
5.6.1.1 | Ni-NTA column, glutathione-agarose beads |
5.6.1.1 | p60, 60 kDa catalytic subunit |
5.6.1.1 | purification of recombinant protein using a glutathione-sepharose column, removing of the GST moiety with precision protease |