EC Number |
---|
5.4.99.11 | - |
5.4.99.11 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephacryl S-300 gel filtration |
5.4.99.11 | expression in Escherichia coli |
5.4.99.11 | native enzyme 17.9fold by adsorption on a chromatographic resin |
5.4.99.11 | Ni-NTA column chromatography |
5.4.99.11 | Ni-NTA column chromatography, gel filtration |
5.4.99.11 | purification and immobilization in a single step |
5.4.99.11 | recombinant enzyme from Escherichia coli strain BL21(DE3) |
5.4.99.11 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
5.4.99.11 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatograhy and gel filtration |