EC Number |
Reference |
---|
5.3.1.14 | - |
2624, 2626, 2627, 2629, 2633, 2637, 652824, 703677, 716819, 727537 |
5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography |
715213 |
5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose ion-exchange chromatography |
727653 |
5.3.1.14 | His-Trap column chromatography |
714573 |
5.3.1.14 | His-Trap HP column chromatography, and gel filtration |
727230 |
5.3.1.14 | native enzyme by anion exchange, hydrophobic interaction, and again anion exchange chromatography to apparent homogeneity, recombinant His6-tagged enzyme 3.87fold from Escherichia coli strain JM109 by nickel affinity chromatography to homogeneity |
677768 |
5.3.1.14 | Ni-NTA column chromatography |
713643, 713898, 714530 |
5.3.1.14 | recombinant |
660751 |
5.3.1.14 | recombinant His-tagged enzyme |
661235 |
5.3.1.14 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis |
677350 |