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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary)
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1-
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1lysate is applied to a chelating sepharose fast flow column charged with Ni2+, the N-terminal His tag is removed by thrombin cleavage
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1mutant enzymes D186A, D186N, D186E
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1Ni-affinity column chromatography and Superdex G75 gel filtration
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1partial
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant cytosolic enzyme
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant His-tagged enzyme
Show all pathways known for 5.1.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.3.1recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and diaylsis
Results 1 - 8 of 8