EC Number |
---|
5.1.3.1 | - |
5.1.3.1 | lysate is applied to a chelating sepharose fast flow column charged with Ni2+, the N-terminal His tag is removed by thrombin cleavage |
5.1.3.1 | mutant enzymes D186A, D186N, D186E |
5.1.3.1 | Ni-affinity column chromatography and Superdex G75 gel filtration |
5.1.3.1 | partial |
5.1.3.1 | recombinant cytosolic enzyme |
5.1.3.1 | recombinant His-tagged enzyme |
5.1.3.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and diaylsis |