EC Number |
---|
5.1.2.2 | - |
5.1.2.2 | by metal ion affinity chromatography |
5.1.2.2 | purification of recombinant MR as a fusion protein with an N-terminal hexahistidine tag using immobilized-nickel ion affinity chromatography and elution with a linear gradient of EDTA |
5.1.2.2 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
5.1.2.2 | recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography and dialysis |
5.1.2.2 | wild type and mutant E317Q |
5.1.2.2 | wild type and mutant K166R |