EC Number   |
|---|
    4.99.1.3 | - |
| 4.99.1.3 | by using a metal chelating affinity column charged with Ni2+ or a chelating Sepharose-column charged with Co2+ |
| 4.99.1.3 | by using metal chelate affinity chromatography |
| 4.99.1.3 | Ni-Sepharose column chromatography |
| 4.99.1.3 | purified anearobically |
| 4.99.1.3 | recombinant His-tagged wild-type isozyme and mutant DELTA28DVU0650 from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration |
| 4.99.1.3 | recombinant His-tagged wild-type isozyme from Escherichia coli by nickel affinity chromatography, dialysis, anion exchange chromatography and gel filtration |
