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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119- 33737, 649144, 649201, 652075, 659723, 697692
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.1190.2% yield,the microsomal isoform elutes at 0.4-0.7 MKCl, at low salt concentrations, whereas the peroxisomal isoform elutes at high salt concentrations 5870
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119recombinant His-tagged MFE-2 from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography, removal of the His tag 714098
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119recombinant His6-tagged PhaJ4 homologues from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 729029
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119recombinant protein purified from the cell extract to apparent homogeneity by three chromatographic steps on anion-exchange, cation-exchange and size-exclusion columns 684952
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) 746834
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119recombinant wild-type and mutant GFP-tagged hydratase domains of multifunctional enzyme type 2 hydratase from Escherichia coli strain JM109 by anion exchange chromatography 730244
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.119the FOX2 gene is overexpressed from a multicopy vector (YEp352) in Saccharomyces cerevisiae and the gene product purified to apparent homogenity. A truncated version of MFP lacking 271 carboxyl-terminal amino acids is also overexpressed and purified 687487
Results 1 - 8 of 8