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Results 1 - 10 of 18 > >>
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13- 693832, 703212, 716329
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13(6-4) photoproduct DNA photolyase activity is detected in Crotalus atrox fibroblast. Activity is considerably enhanced when a UV-damaged DNA affinity column is used for purification. However, the activity is unstable and it is lost during purification or upon storage at -20° or -70°C for 2-3 months 682264
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13by fractionation of crude cell extracts with Heparin agarose and UV DNA affinity column chromatography 682086
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13by using a glutathione sepharose column and a Hi Trap Q column. Concentrated fusion protein is cleaved with thrombin from bovine plasma by incubation overnight at 4°C. For chromophore determination, the eluate from the glutathione sepharose column is purified through a Q sepharose column, omitting gel filtration procedure, and concentrated by ultrafiltration 682048
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13by using a glutathione-sepharose column and UV-irradiated DNA affinity column, fusion protein is cleaved with thrombin 682154
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13by using affinity chromatography and UV-irradiated DNA attached beads 682855
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13by using glutathione-sepharose columns 679920, 680605
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13fusion protein is applied to amylose column. At this point the protein is above 90% pure. Further purification can be obtained by applying the eluted material to a 10 ml heparin-agarose column. Maltose-binding protein is removed by treatment with factor Xa protease 681765
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13fusion protein is purified through a 20-ml amylose column and through heparin-agarose column 680604
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.13NF-1 is isolated from nuclear extracts prepared from HeLa cell culture using a heparin column, an anion exchange Fractogel EMD TMAE-650 (S) column and a FPLC MonoS HR5/5 column 682153
Results 1 - 10 of 18 > >>