EC Number |
---|
4.1.3.36 | - |
4.1.3.36 | ammonium sulfate precipitation, ion exchange chromatography (DEAE), gel filtration |
4.1.3.36 | His-tag affinity chromatography, tag removed |
4.1.3.36 | HisTrap Chelating HP5 Superdex 200 gel filtration and HiLoad 16/60 Superdex 200 gel filtration |
4.1.3.36 | HiTrap chelating column chromatography and Sephacryl S-100 gel filtration |
4.1.3.36 | HiTrap Chelating HP column chromatography and HiTrap column chromatography |
4.1.3.36 | recombinant enzyme |
4.1.3.36 | recombinant enzyme from Escherichia coli strain BL21(DE3) too over 95% purity |
4.1.3.36 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) too over 95% purity |