EC Number |
---|
3.6.1.10 | - |
3.6.1.10 | DEAE Toyopearl 650M column chromatography, heparin agarose column chromatography, and Mono Q column chromatography |
3.6.1.10 | native enzyme from exopolyphosphatase-deficient mutant strain CRX by heparin affinity and phosphocellulose chromatography, 3820fold to homogeneity |
3.6.1.10 | recombinant enzyme DPP1 from Saccharomyces cerevisiae strain CRN by anion exchange chromatography and ultrafiltration |
3.6.1.10 | recombinant enzyme PPN1 from Saccharomyces cerevisiae strain CRN by ammonium sulfate fractionation, anion exchange chromatography, heparin affinity chromatography, all alternating with ultrafiltration steps |
3.6.1.10 | recombinant enzyme PPN2 from Saccharomyces cerevisiae strain CRN by ammonium sulfate fractionation, anion exchange chromatography, heparin affinity chromatography, all alternating with ultrafiltration steps |
3.6.1.10 | recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and dialysis |