EC Number   |
|---|
    3.5.99.6 | - |
| 3.5.99.6 | ammonium sulfate precipitation, Resource Q column chromatography, and Ni2+-IDA agarose column chromatography |
| 3.5.99.6 | N-6-aminohexanoyl-glucosamine 6-phosphate agarose column chromatography |
| 3.5.99.6 | native enzyme 1390fold from kidney cortex by affinity chromatography on N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose and on phosphocellulose, the latter behaving as an active site affinity resin |
| 3.5.99.6 | native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on a N-aminohexyl-glucosamine-6-phosphate resin folowed by dialysis and ultrafiltration |
| 3.5.99.6 | recombinant enzyme |
| 3.5.99.6 | recombinant enzyme NagB from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration, to near homogeneity |
| 3.5.99.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity in two steps by nickel affinity chromatography and gel filtration |
| 3.5.99.6 | recombinant isozyme GNPDA1 from Escherichia coli strain IBPC 590 by single purification step by means of allosteric-site affinity chromatography on N-6-aminohexanoyl-GlcN6P agarose |
| 3.5.99.6 | wild-type enzyme and two mutants |
