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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 3.5.4.27Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.27-
Show all pathways known for 3.5.4.27Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.27recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and ion exchange chromatography, native enzyme over 600fold from cell supernatant by ammonium sulfate fractionation and two steps of ion exchange chromatography, purification requires anaerobic conditions, the enzyme is completely soluble at 80% saturated ammonium sulfate
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