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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 3.5.4.25Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.25- 246729, 246730, 246731, 246732, 246734
Show all pathways known for 3.5.4.25Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.25ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-200 gel filtration 726585
Show all pathways known for 3.5.4.25Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.25DEAE-Sephadex column chromatography, Q-Sepharose column chromatography, butyl-Sepharose column chromatography, and Sephacryl S-300 gel filtration 685065
Show all pathways known for 3.5.4.25Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.25purification of wild type enzyme and its mutants by nickel affinity chromatography, site-directed variants are expressed in the native state and purified to homogeneity by ion exchange and hydrophobic interaction chromatographic steps 696250
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