EC Number |
---|
3.5.1.75 | native enzyme by ultrasonication, concentration by PEG 20000, gel filtration, and ultrafilatration |
3.5.1.75 | native enzyme to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography |
3.5.1.75 | native urethanase 3.1fold by ethanol precipitation, and anion exchange chromatography |
3.5.1.75 | partial |
3.5.1.75 | recombinant His-tagged enzyme, refolded from Escherichia coli strain BL21(DE3) inclusion bodies, 1.22fold by nickel affinity chromatography |
3.5.1.75 | strain IFO 12107 |