EC Number   |
|---|
    3.5.1.4 | - |
| 3.5.1.4 | a. transformant : sonication, lysis, dialysis against urea, b. wild-type amidase: dialysis, Mono-Q HR 5/5 column, ammonium sulfate precipitation, dialysis |
| 3.5.1.4 | acetone precipitation, ammonium sulfate fractionation, DEAE-Sepharose chromatography, Mono Q column and gel filtration, purified approximately 138fold with a yield of 20% |
| 3.5.1.4 | acetone precipitation, DEAE-Sepharose, ammonium sulfate precipitation, yield 10% |
| 3.5.1.4 | affinity chromatography on an acetamide epoxy-activated Sepharose column |
| 3.5.1.4 | affinity chromatography on epoxy-activated Sepharose 6B-acetamide and gel filtration |
| 3.5.1.4 | ammonium sulfate precipitation and dialysis and gel filtration on DEAE-Sephadex A-50 |
| 3.5.1.4 | ammonium sulfate precipitation, 30 to 55%, and hydrophobic, anion-exchange, gel filtration, and ceramic hydroxyapatite chromatography steps, DTT required to maintain enzymatic activity, overall level of purification 114-fold, recovery rate 21% |
| 3.5.1.4 | cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column |
| 3.5.1.4 | dialysis, column chromatography and ammonium sulfate fractionation |
