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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17- 670041, 712896
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17coiled-coil C-terminus, residues 541-585, as His-tagged peptide 652822
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17HiTrapQ column chromatography and Superdex 200 gel filtration 681654
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17Ni-NTA agarose column chromatography, and gel filtration 734211
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant enzyme, solubilization by N-lauylsarcosine, refolding in presence of ATP, Mg2+ and Zn2+, and purification to near homogeneity 650184
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant Hi-Myc-tagged enzyme 652391
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant His-Myc-tagged wild-type and mutant enzymes 650082
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant His-Myc-tagged wild-type and mutants 651856
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant His-tagged enzyme from Escherichia coli 651768
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.B17recombinant soluble fusion proteins, more than 80% homogeneity 651260
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