3.4.24.34 | recombinant His-tagged thioredoxin-S-fusion enzyme from Escherichia coli strain BL21 by nicke affinity chromatography, ultrafiltration, tag cleavage by thrombin, followed by gel filtration, recombinant His-/claMP-tagged enzyme is purified by nicel affinity chromatography, tag cleavage by factor Xa, and gel filtration. Reaction with Factor Xa leads to incomplete cleavage but the uncut fusion protein is easily removed by gel filtration |
755297 |