EC Number   |
|---|
   3.4.24.13 | - |
| 3.4.24.13 | Ni-NTA column chromatography |
| 3.4.24.13 | partial |
| 3.4.24.13 | recombinant glutathione S-transferase fusion proteins of three segments of enzyme, comprising amino acids 1032-1964, 708-1964, and 104-1964. Segment 1032-1964 is catalytically inactive. Recombinant proteins are highly instable. |
| 3.4.24.13 | recombinant N-terminally tagged wild-type and mutant active mature enzymes from Escherichia coli strain BL21(DE3) by nickel and amylose affinity chromatography, MBP-tag cleavage through TEV and the His-tag through thrombin, and gel filtration. Recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography an, tag cleavage through thrombin, and gel filtration |
| 3.4.24.13 | type 1 enzyme |
| 3.4.24.13 | type 2 enzyme |
| 3.4.24.13 | using Ni-NTA agorose binding |
