EC Number   |
Reference |
|---|
   3.4.22.65 | - |
647685, 665200, 677434, 680857, 695433, 698444, 699570 |
| 3.4.22.65 | affinity chromatography |
664637 |
| 3.4.22.65 | ammonium sulfate precipitation, gel filtration, ion exchange chromatography |
708179 |
| 3.4.22.65 | anion exchange and gel filtration |
665290 |
| 3.4.22.65 | column chromatography |
709362 |
| 3.4.22.65 | culture supernatants recovered, buffer exchange by gel filtration, equilibration with 100 mM sodium acetate buffer (pH 4) |
665201 |
| 3.4.22.65 | dialysis, cationic exchange on SP-Sepharose, recombinant Der p 1 dialysis, benzamidine column, dialysis |
665292 |
| 3.4.22.65 | immunoaffinity chromatography using immobilized 4C1 anti-Der p 1 monoclonal antibody |
665740 |
| 3.4.22.65 | mature form purified by anion exchange and gel filtration chromatography |
665200 |
| 3.4.22.65 | mature forms were purified by anion-exchange and gel filtration chromatography, proforms were purified by means of the same methods as the mature forms but without activation by dialysis against acidic buffer, natural Der f 1 was purified from whole culture extracts of Dermatophagoides farinae |
665290 |
