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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35- 30316, 30320, 30323, 30325
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.3515fold, to homogeneity 651056
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35Ni-Sepharose column chromatography 731356
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35Ni2+-NTA resin column chromatography, and gel filtration 732864
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35partial 30319
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35recombinant His-tagged enzyme from Escherichia coli 653261
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, from Escherichia coli strain BL21 by nickel affinity chromatography 668210
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35recombinant His-tagged inactive pro-enzyme to homogeneity 653742
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35recombinant protein using His-tag under denaturating conditions 683417, 683468
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.35recombinant refolded His-tagged enzyme by nickel affinity chromatography 701062
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