EC Number   |
Reference |
|---|
   3.4.22.2 | - |
649434, 678334, 718346, 81552, 95675, 95683, 95707 |
| 3.4.22.2 | active papain is prepared from the commercial papaya latex enzyme through affinity purification on Sepharose 4B |
697918 |
| 3.4.22.2 | ammonium sulfate precipitation |
681479 |
| 3.4.22.2 | aqueous two-phase system (40% (w/w) 15 mg/ml enzyme solution, 14.33-17.65% (w/w) PEG 6000, 14.27-14.42% (w/w) NaH2PO4/K2HPO4 and pH 5.77-6.3 at 20°C). Propanol generates solid enzyme aggregates with almost 120% activity upon resolubilization through dilution of the precipitant |
718263 |
| 3.4.22.2 | further purification of the commercial enzyme preparation from latex by gel filtration, to homogeneity |
667511 |
| 3.4.22.2 | insoluble His-tagged fusion protein is solubilized and purified by nickel chelate affinity chromatography under denaturing conditions and it is further purified by gel filtration chromatography |
700680 |
| 3.4.22.2 | native enzyme from latex |
753097 |
| 3.4.22.2 | purification of papain from papaya powder extracts by membranes immobilized with Reactive Red 120 or Reactive Brown 10 as dye ligands. Papain adsorption capacities for the Red 120 and Brown 10 membranes are 143.6 mg/g and 107.3 mg/g, respectively. Yields of over 80% are found for the Red 120-chitosan-nylon membrane whereas only a 50% recovery is possible using the Brown 10-CS-nylon membranes |
708210 |
| 3.4.22.2 | purification of papain using the dye affinity membrane chromatography at pH 7.05 with Tris-HCl, papain is purified 34.6fold in a single step determined by fast protein liquid chromatography |
696789 |
| 3.4.22.2 | recombinant enzyme |
95699 |
