EC Number |
---|
3.4.21.25 | - |
3.4.21.25 | 40fold |
3.4.21.25 | 60% ammonium sulfate precipitation with 1.0:1.25 ratio of crude extract:t-butanol at pH and temperature of 8.0 and 20°C |
3.4.21.25 | a quick purification method leads to an enzyme fraction with almost only the 67000 Da enzyme |
3.4.21.25 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration |
3.4.21.25 | DEAE-Sepharose chromatography, CM-Sepharose chromatography and ammonium sulfate precipitation |
3.4.21.25 | gel filtration, SDS-PAGE |
3.4.21.25 | HiTrap chelating column chromatography and Superdex 200 gel filtration |
3.4.21.25 | HiTrap SP column chromatography and Superdex 200 gel filtration |
3.4.21.25 | Ni-NTA affinity column chromatography |