   3.4.14.11 | native enzyme, comparison of the effects of several cell disruption methods on the activity of PepX, detailed overview. Statistical optimization methods are performed for two cavitation methods, hydrodynamic (high-pressure homogenization) and acoustic (sonication), to determine the more appropriate disruption method. Two level factorial design (2FI), with the parameters of number of cycles and pressure, and Box-Behnken design (BBD), with the parameters of cycle, sonication time, and power, are used for the optimization of the high-pressure homogenization and sonication methods, respectively. In addition, disruption methods, consisting of lysozyme, bead milling, heat treatment, freeze-thawing, liquid nitrogen, EDTA, Triton-X, SDS, chloroform, and antibiotics, are performed and compared with the high-pressure homogenization and sonication methods. The optimized values of high-pressure homogenization are one cycle at 130 MPa providing activity of 114.47 mU/ml, while sonication afforded an activity of 145.09 mU/ml at 28 min with 91% power and three cycles |
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