EC Number |
---|
3.4.13.19 | - |
3.4.13.19 | 2 forms different in electrophoretic mobility: form S (slow) and F (fast) |
3.4.13.19 | affinity purification of enzyme solubilized with detergent |
3.4.13.19 | ammonium sulfate precipitation, size exclusion chromatography, ion exchange chromatography and gel filtration |
3.4.13.19 | partial |
3.4.13.19 | recombinant enzyme is purified by nickel-chelating affinity chromatography, wild type enzyme is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Q-Sepharose column chromatography |
3.4.13.19 | Sephadex G-25 gel filtration, Toyopearl Butyl-650M column chromatography, Toyopearl DEAE-650M column chromatography, and Sephacryl S-200 gel filtration |
3.4.13.19 | ultracentrifugation and Superose 12 (10/300) gel filtration |